Coding

Part:BBa_K4343111

Designed by: Yue Shu   Group: iGEM22_NNU-China   (2022-10-09)


rElo2

rElo2 encodes C16/18 elongase from rat which functions in the desaturase and elongase pathway and could convert C16:0 to C18:0.

Description

The wild-type Y. lipolytica Po1f can produce C18:0 under the catalysis of C16/18 elongase. rElo2 encodes C16/18 elongase of 267 amino acids from rat. Heterologous expression of rElo2 could catalyze C16:0 to C18:0 in Y. lipolytica.

Literature

Some studies have used C16/18 elongase from this source to produce high yields of omega-3 fatty acids.

Reference

Yazawa, H., Kamisaka, Y., Kimura, K., Yamaoka, M., & Uemura, H. (2011). Efficient accumulation of oleic acid in Saccharomyces cerevisiae caused by expression of rat elongase 2 gene (rELO2) and its contribution to tolerance to alcohols. Applied microbiology and biotechnology, 91(6), 1593-1600.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 288
    Illegal BsaI.rc site found at 821
    Illegal SapI site found at 749


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Parameters
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